Principle:- Nutrient broth high grow is a modified media and commonly used for cultivation, enrichment, enumeration, prolonged maintenance and storage of wide variety of microbes. The nutrient broth HG is widely used as non-selective microbial media for routine microbiology analysis and cultivation of non-fastidious organisms. Nutrient broth HG is ideal microbial media for cultivation of commercially important microorganism were high biomass is required. Nutrient Broth HG is a simple medium composed of the special peptone, special meat extract, yeast extract and sodium chloride. Special peptone, special meat extract and yeast extract provide ample amount of the carbon and nitrogen essential and non-essential amino acids, along with the vitamins and trace minerals for the growth of microorganisms. Sodium chloride maintains the osmotic equilibrium of the medium. Directions:- Nutrient Broth (HG) Dissolve 13.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
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